Expression of complement components, receptors and regulators by human dendritic cells

Purification of complement components, regulators, and receptors by classical methods.

Human Leukocyte Antigen-G Expression on Dendritic Cells Induced by Transforming Growth Factor-β1 and CD4+ T Cells Proliferation

Background: During antigen capture and processing, mature dendritic cells (DC) express large amounts of peptide-MHC complexes and accessory molecules on their surface. DC are antigen-presenting cells that have an important role in tolerance and autoimmunity. The transforming growth factor-beta1 (TGF-β1) cytokine has a regulatory role on the immune and non-immune cells. The aim of this study is ...

Adiponectin Effect on The Viability of Human Endometrial Stromal Cells and mRNA Expression of Adiponectin Receptors

Enhanced expression of human monocyte complement (C3b) receptors by chemoattractants.

The capacity of various leucoattractants to enhance, or unfold, receptors for complement (C3b) on human blood monocytes has been studied. A number of recognized monocyte chemoattractants including casein, supernatants from C. parvum 10390 and from human lymphocytes (cultured either in the presence or absence of phytohaemagglutinin) and the formyl-methionyl peptides, F-Met-Leu-Phe, F-Met-Met-Phe...

Expression of multilectin receptors and comparative FITC-dextran uptake by human dendritic cells.

Dendritic cells (DC) are potent antigen-presenting cells and understanding their mechanisms of antigen uptake is important for loading DC with antigen for immunotherapy. The multilectin receptors, DEC-205 and macrophage mannose receptor (MMR), are potential antigen-uptake receptors; therefore, we examined their expression and FITC-dextran uptake by various human DC preparations. The RT-PCR anal...